Cellosaurus HeLa EGFP-654 (CVCL_VS42)
| Cell line name | HeLa EGFP-654 |
|---|---|
| Accession | CVCL_VS42 |
| Resource Identification Initiative | To cite this cell line use: HeLa EGFP-654 (RRID:CVCL_VS42) |
| Comments | Population: African American. Characteristics: Transfected with a construct containing a mutated human beta-globin intron, HBB IVS2-654, at nucleotide 105 of EGFP cDNA thus preventing its correct translation. Treatment of the cells expressing the construct with active antisense oligonucleotide should restore proper splicing and translation of EGFP, providing a rapid and sensitive positive readout for antisense activity in the nuclei of the treated cells (PubMed=11574678; PubMed=11748845). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
| Disease | Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0058 (HeLa S3) |
| Sex of cell | Female |
| Age at sampling | 30Y6M |
| Category | Cancer cell line |
| Web pages | Provider; UNC OTC; 16-0176; https://unc.flintbox.com/technologies/2512ea18-bd35-4199-8769-0c6bd96cbcdc |
| Publications | PubMed=11574678; DOI=10.1093/nar/29.19.3965; PMCID=PMC60237 PubMed=11748845; DOI=10.1002/humu.1229 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q94096364 |
| Entry history | |
| Entry creation | 25-Feb-2019 |
| Last entry update | 10-Apr-2025 |
| Version number | 8 |