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Cellosaurus U2OS#24 (CVCL_VR87)

[Text version]
Cell line name U2OS#24
Synonyms U2OS clone #24; U2OS reporter cell line #24
Accession CVCL_VR87
Resource Identification Initiative To cite this cell line use: U2OS#24 (RRID:CVCL_VR87)
Comments Population: Caucasian.
Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=15590323; DOI=10.1016/j.dnarep.2004.08.010; PMCID=PMC2967438
Puget N., Knowlton M., Scully R.
Molecular analysis of sister chromatid recombination in mammalian cells.
DNA Repair 4:149-161(2005)

Cross-references
Encyclopedic resources Wikidata; Q98133787
Entry history
Entry creation25-Feb-2019
Last entry update10-Sep-2024
Version number9