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Cellosaurus HEK293T GALE+GALK2 KO clone 7 (CVCL_VR15)

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Cell line name HEK293T GALE+GALK2 KO clone 7
Accession CVCL_VR15
Resource Identification Initiative To cite this cell line use: HEK293T GALE+GALK2 KO clone 7 (RRID:CVCL_VR15)
Comments Characteristics: Devoid of all O-glycosylation in all culture condition.
Knockout cell: Method=CRISPR/Cas9; HGNC; 4116; GALE.
Knockout cell: Method=CRISPR/Cas9; HGNC; 4119; GALK2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VR11 (HEK293T GALE KO clone 4)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=28654657; DOI=10.1371/journal.pone.0179949; PMCID=PMC5487050
Termini J.M., Silver Z.A., Connor B., Antonopoulos A., Haslam S.M., Dell A., Desrosiers R.C.
HEK293T cell lines defective for O-linked glycosylation.
PLoS ONE 12:E0179949-E0179949(2017)

Cross-references
Cell line collections (Providers) Kerafast; EMI018
Encyclopedic resources Wikidata; Q94095770
Entry history
Entry creation13-Nov-2018
Last entry update10-Sep-2024
Version number8