Cellosaurus HEK293T GALE KO clone 4 (CVCL_VR11)
| Cell line name | HEK293T GALE KO clone 4 | ||
|---|---|---|---|
| Accession | CVCL_VR11 | ||
| Resource Identification Initiative | To cite this cell line use: HEK293T GALE KO clone 4 (RRID:CVCL_VR11) | ||
| Comments | Characteristics: Devoid of all O-glycosylation. But with the addition of galactose and GalNAc to the culture media, salvage pathways are able to compensate for the lack of GALE enzymatic activity and restore O-linked glycosylation. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:4116; GALE. Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. | ||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||
| Hierarchy | Parent: CVCL_0063 (HEK293T) Children:
| ||
| Sex of cell | Female | ||
| Age at sampling | Fetus | ||
| Category | Transformed cell line | ||
| Publications | PubMed=28654657; DOI=10.1371/journal.pone.0179949; PMCID=PMC5487050 | ||
| Cross-references | |||
| Cell line collections (Providers) | Kerafast; EMI014 | ||
| Encyclopedic resources | Wikidata; Q94095755 | ||
| Entry history | |||
| Entry creation | 13-Nov-2018 | ||
| Last entry update | 19-Dec-2024 | ||
| Version number | 9 | ||