Cellosaurus CRISPRi Gen1B 1 (CVCL_VM35)
Cell line name | CRISPRi Gen1B 1 |
---|---|
Synonyms | WTB CRISPRi Gen1 clone 1; 1B-1 |
Accession | CVCL_VM35 |
Resource Identification Initiative | To cite this cell line use: CRISPRi Gen1B 1 (RRID:CVCL_VM35) |
Comments | Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi). Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus. Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_VM30 (WTB6) |
Sex of cell | Female |
Age at sampling | Age unspecified |
Category | Induced pluripotent stem cell |
Publications | PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697 |
Cross-references | |
Encyclopedic resources | Wikidata; Q93458758 |
Entry history | |
Entry creation | 07-Sep-2018 |
Last entry update | 10-Sep-2024 |
Version number | 7 |