ID   CRISPRi Gen1B 1
AC   CVCL_VM35
SY   WTB CRISPRi Gen1 clone 1; 1B-1
DR   Wikidata; Q93458758
RX   PubMed=26971820;
CC   Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC   Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
CC   Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_VM30 ! WTB6
SX   Female
AG   Age unspecified
CA   Induced pluripotent stem cell
DT   Created: 07-09-18; Last updated: 10-09-24; Version: 7
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RX   PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697;
RA   Mandegar M.A., Huebsch N., Frolov E.B., Shin E., Truong A.,
RA   Olvera M.P., Chan A.H., Miyaoka Y., Holmes K., Spencer C.I.,
RA   Judge L.M., Gordon D.E., Eskildsen T.V., Villalta J.E., Horlbeck M.A.,
RA   Gilbert L.A., Krogan N.J., Sheikh S.P., Weissman J.S., Qi L.S.,
RA   So P.-L., Conklin B.R.;
RT   "CRISPR interference efficiently induces specific and reversible gene
RT   silencing in human iPSCs.";
RL   Cell Stem Cell 18:541-553(2016).
//