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Cellosaurus HUDEP-2 (CVCL_VI06)

[Text version]
Cell line name HUDEP-2
Synonyms HUDEP2; Human Umbilical cord blood-Derived Erythroid Progenitor-2
Accession CVCL_VI06
Resource Identification Initiative To cite this cell line use: HUDEP-2 (RRID:CVCL_VI06)
Comments Population: Japanese.
Characteristics: After in vitro induction of differentiation, will produce enucleated red blood cells.
Characteristics: Expression of the HPV16 E6 and E7 genes is induced by tetracycline (PubMed=23533656).
Doubling time: 22.7 +- 5.0 hours (PubMed=31753923).
Genetic integration: Method=Transduction; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: Chromatin accessibility by ATAC-seq.
Omics: Deep quantitative proteome analysis.
Omics: Transcriptome analysis by RNAseq.
Miscellaneous: Please contact directly Dr. Nakamura Y. for the use of this cell line (yukionak@brc.riken.jp).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Cell type: Erythroid progenitor cell; CL=CL_0000038.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C0QM (HUDEP2 HBB-KO)CVCL_YT59 (ssHUDEP2)
Sex of cell Sex unspecified
Age at sampling <1D
Category Transformed cell line
Publications

PubMed=23533656; DOI=10.1371/journal.pone.0059890; PMCID=PMC3606290
Kurita R., Suda N., Sudo K., Miharada K., Hiroyama T., Miyoshi H., Tani K., Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS ONE 8:E59890-E59890(2013)

Patent=US8975072
Nakamura Y., Kurita R.
Human erythroid progenitor cell line comprising HPV E6/E7 operably linked to an inducible promoter and method for producing human enucleated red blood cells.
Patent number US8975072, 10-Mar-2015

PubMed=31164570; DOI=10.3390/mps1030028; PMCID=PMC6481050
Moir-Meyer G., Cheong P.L., Olijnik A.-A., Brown J., Knight S., King A., Kurita R., Nakamura Y., Gibbons R.J., Higgs D.R., Buckle V.J., Babbs C.
Robust CRISPR/Cas9 genome editing of the HUDEP-2 erythroid precursor line using plasmids and single-stranded oligonucleotide donors.
Methods Protoc. 1:28.1-28.17(2018)

PubMed=31753923; DOI=10.3324/haematol.2019.229211; PMCID=PMC7395286
Daniels D.E., Downes D.J., Ferrer-Vicens I., Ferguson D.C.J., Singleton B.K., Wilson M.C., Trakarnsanga K., Kurita R., Nakamura Y., Anstee D.J., Frayne J.
Comparing the two leading erythroid lines BEL-A and HUDEP-2.
Haematologica 105:e389-e394(2020)

PubMed=35269949; DOI=10.3390/ijms23052807; PMCID=PMC8911073
Zhou G.-Q., Zhang H.-K., Lin A.-N., Wu Z., Li T., Zhang X.-M., Chen H.-Y., Lu D.-R.
Multi-omics analysis in beta-thalassemia using an HBB gene-knockout human erythroid progenitor cell model.
Int. J. Mol. Sci. 23:2807.1-2807.14(2022)

Cross-references
Cell line collections (Providers) RCB; RCB4557
CRISP screens repositories BioGRID_ORCS_Cell_line; 1800
Encyclopedic resources Wikidata; Q54896730
Proteomic databases PRIDE; PXD030182
Entry history
Entry creation14-May-2018
Last entry update10-Sep-2024
Version number15