Cellosaurus cell line MCF7 Tet-On (CVCL

Cellosaurus MCF7 Tet-On (CVCL_V332)

[Text version]

Cell line name

MCF7 Tet-On

Synonyms

MCF7 Tet On; MCF-7 Tet-On

Accession

CVCL_V332

Resource Identification Initiative

To cite this cell line use: MCF7 Tet-On (RRID:CVCL_V332)

Comments

Population: Caucasian.
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Discontinued: Takara/Clontech; Catalog number 630918.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).

Disease

Invasive breast carcinoma of no special type (NCIt: C4194)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0031 (MCF-7)
Children:

CVCL_JX63 (MCF7EL)

CVCL_JX64 (MCF7T1)

CVCL_JX65 (MCF7T2)

Sex of cell

Female

Age at sampling

69Y