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Cellosaurus U-87MG ATCC IDH1 p.R132H-Luc2 (CVCL_UR35)

[Text version]
Cell line name U-87MG ATCC IDH1 p.R132H-Luc2
Synonyms IDH1 Mutant U-87 Isogenic-Luc2
Accession CVCL_UR35
Resource Identification Initiative To cite this cell line use: U-87MG ATCC IDH1 p.R132H-Luc2 (RRID:CVCL_UR35)
Comments Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.
Population: Caucasian.
Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter.
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=With optimized codon usage for mammalian expression).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Sequence variations
  • Mutation; HGNC; HGNC:5382; IDH1; Simple_edited; p.Arg132His (c.395G>A); ClinVar=VCV000156444; Zygosity=Heterozygous; Note=By CRISPR/Cas9 (ATCC=HTB-14IG-LUC2).
  • Mutation; HGNC; HGNC:7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UE09 (U-87MG ATCC IDH1 p.R132H)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): ATCC=HTB-14IG-LUC2

Markers:
AmelogeninX
CSF1PO10,11
D5S81811,12
D7S8208,9
D13S3178,11
D16S53912
TH019.3
TPOX8
vWA15,17

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Cross-references
Cell line collections (Providers) ATCC; HTB-14IG-LUC2
Cell line databases/resources cancercelllines; CVCL_UR35
Encyclopedic resources Wikidata; Q98133709
Entry history
Entry creation25-Feb-2019
Last entry update19-Dec-2024
Version number12