ID   U-87MG ATCC IDH1 p.R132H-Luc2
AC   CVCL_UR35
SY   IDH1 Mutant U-87 Isogenic-Luc2
DR   ATCC; HTB-14IG-LUC2
DR   cancercelllines; CVCL_UR35
DR   Wikidata; Q98133709
CC   Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.
CC   Population: Caucasian.
CC   Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter.
CC   Sequence variation: Mutation; HGNC; 5382; IDH1; Simple_edited; p.Arg132His (c.395G>A); ClinVar=VCV000156444; Zygosity=Heterozygous; Note=By CRISPR/Cas9 (ATCC).
CC   Sequence variation: Mutation; HGNC; 7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Transfected with: UniProtKB; P08659; Firefly luciferase (with optimized codon usage for mammalian expression).
ST   Source(s): ATCC
ST   Amelogenin: X
ST   CSF1PO: 10,11
ST   D13S317: 8,11
ST   D16S539: 12
ST   D5S818: 11,12
ST   D7S820: 8,9
ST   TH01: 9.3
ST   TPOX: 8
ST   vWA: 15,17
DI   NCIt; C3058; Glioblastoma
DI   ORDO; Orphanet_360; Glioblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_UE09 ! U-87MG ATCC IDH1 p.R132H
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 05-10-23; Version: 9
//