Cellosaurus HeLa GFP-LC3-RFP-LC3deltaG (CVCL_UP51)
| Cell line name | HeLa GFP-LC3-RFP-LC3deltaG |
|---|---|
| Synonyms | GFP-LC3-RFP-LC3deltaG HeLa |
| Accession | CVCL_UP51 |
| Resource Identification Initiative | To cite this cell line use: HeLa GFP-LC3-RFP-LC3deltaG (RRID:CVCL_UP51) |
| Comments | Population: African American. Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG. Genetic integration: Method=Transfection/transduction; Gene=RGD; 735183; Map1lc3a. Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
| Disease | Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0030 (HeLa) |
| Sex of cell | Female |
| Age at sampling | 30Y6M |
| Category | Cancer cell line |
| Publications | PubMed=27818143; DOI=10.1016/j.molcel.2016.09.037 |
| Cross-references | |
| Cell line collections (Providers) | RCB; RCB4695 |
| Encyclopedic resources | Wikidata; Q94096465 |
| Entry history | |
| Entry creation | 25-Feb-2019 |
| Last entry update | 10-Sep-2024 |
| Version number | 9 |