ID   HeLa GFP-LC3-RFP-LC3deltaG
AC   CVCL_UP51
SY   GFP-LC3-RFP-LC3deltaG HeLa
DR   RCB; RCB4695
DR   Wikidata; Q94096465
RX   PubMed=27818143;
CC   Population: African American.
CC   Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG.
CC   Transfected with: RGD; 735183; Map1lc3a.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: mRFP1, a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 25-02-19; Last updated: 02-05-24; Version: 8
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RX   PubMed=27818143; DOI=10.1016/j.molcel.2016.09.037;
RA   Kaizuka T., Morishita H., Hama Y., Tsukamoto S., Matsui T., Toyota Y.,
RA   Kodama A., Ishihara T., Mizushima T., Mizushima N.;
RT   "An autophagic flux probe that releases an internal control.";
RL   Mol. Cell 64:835-849(2016).
//