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Cellosaurus hTERT NF1 ipNF05.5 (CVCL_UI71)

[Text version]
Cell line name hTERT NF1 ipNF05.5
Synonyms ipNF05.5
Accession CVCL_UI71
Resource Identification Initiative To cite this cell line use: hTERT NF1 ipNF05.5 (RRID:CVCL_UI71)
Comments Population: Caucasian.
Doubling time: ~59 hours (ATCC=CRL-3388).
Karyotypic information: 46,XY,t(2;3)(q23;p26) [18]; 47,sl,+9 [2] (ATCC=CRL-3388).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by RNAseq.
Caution: This cell line was also termed 'ipNF05.5 (single clone)', but this is a misnomer as this cell line is not clonal (personal communication of Allaway, Robert J.).
Derived from site: In situ; Peripheral nervous system, nerve; UBERON=UBERON_0001021.
Cell type: Schwann cell; CL=CL_0002573.
Sequence variations
  • Gene deletion; HGNC; HGNC:7765; NF1; Zygosity=Heterozygous; Note=Somatic LOH (PubMed=27617404).
  • Mutation; HGNC; HGNC:7765; NF1; Simple; p.Leu1153Thrfs*42 (c.3456dupA) (3456_3457insA); ClinVar=VCV000656975; Zygosity=Heterozygous; Note=Germline (PubMed=27617404).
Disease Neurofibromatosis type 1 (NCIt: C3273)
Neurofibromatosis type 1 (ORDO: Orphanet_636)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_UI72 ! hTERT NF1 ipNF05.5 (Mixed clones)
CVCL_UI73 ! hTERT NF1 ipnNF09.4
Sex of cell Male
Age at sampling 22Y
Category Telomerase immortalized cell line
STR profile Source(s): ATCC=CRL-3388; PubMed=27617404

Markers:
AmelogeninX,Y
CSF1PO10,11
D2S133817
D3S135814,17
D5S81812
D7S8208,12
D8S117913,14
D13S3178,12
D16S53911,12
D18S5115,19
D19S43314,16
D21S1130
FGA21
Penta D9,13
Penta E5,10
TH016,9.3
TPOX8,12
vWA17,18

Run an STR similarity search on this cell line
Web pages http://www.n-tap.org/plexiform-neurofibromas/cell-lines/
Publications

PubMed=27617404; DOI=10.1038/labinvest.2016.88
Li H., Chang L.-J., Neubauer D.R., Muir D.F. 4th, Wallace M.R.
Immortalization of human normal and NF1 neurofibroma Schwann cells.
Lab. Invest. 96:1105-1115(2016)

PubMed=29893754; DOI=10.1038/sdata.2018.106; PMCID=PMC5996849
Ferrer M., Gosline S.J.C., Stathis M., Zhang X.-H., Guo X.-D., Guha R., Ryman D.A., Wallace M.R., Kasch-Semenza L., Hao H.-P., Ingersoll R.G., Mohr D., Thomas C.J., Verma S., Guinney J.H., Blakeley J.O.
Pharmacological and genomic profiling of neurofibromatosis type 1 plexiform neurofibroma-derived Schwann cells.
Sci. Data 5:180106-180106(2018)

Cross-references
Cell line collections (Providers) ABM; T0180
ATCC; CRL-3388
Encyclopedic resources Wikidata; Q94305827
Entry history
Entry creation25-Feb-2019
Last entry update19-Dec-2024
Version number15