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Cellosaurus HT1080-iRFP-Puro (CVCL_RA17)

[Text version]
Cell line name HT1080-iRFP-Puro
Accession CVCL_RA17
Resource Identification Initiative To cite this cell line use: HT1080-iRFP-Puro (RRID:CVCL_RA17)
Comments Population: Caucasian.
Characteristics: Reporter cell line, transduced with the LV-iRFP-P2A-Puro vector encoding for iRFP linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL077).
Genetic integration: Method=Transduction; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0317 (HT-1080)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL077
Cell line databases/resources cancercelllines; CVCL_RA17
Encyclopedic resources Wikidata; Q54896565
Entry history
Entry creation05-Mar-2018
Last entry update19-Dec-2024
Version number15