Cellosaurus HT1080-hNIS-Neo/iRFP-Puro (CVCL_RA15)
| Cell line name | HT1080-hNIS-Neo/iRFP-Puro |
|---|---|
| Accession | CVCL_RA15 |
| Resource Identification Initiative | To cite this cell line use: HT1080-hNIS-Neo/iRFP-Puro (RRID:CVCL_RA15) |
| Comments | Population: Caucasian. Characteristics: Reporter cell line, transfected with both the LV-hNIS-IRES-Neo and LV-iRFP-P2A-Puro vectors. LV-hNIS-IRES-Neo encodes for human SLC5A5 linked via an internal ribosomal entry site (IRES) to the Neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-iRFP-P2A-Puro encodes for iRFP linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL107). Genetic integration: Method=Transfection; Gene=HGNC; 11040; SLC5A5. Genetic integration: Method=Transfection; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269. |
| Sequence variations |
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| Disease | Fibrosarcoma (NCIt: C3043) Fibrosarcoma (ORDO: Orphanet_2030) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0317 (HT-1080) |
| Sex of cell | Male |
| Age at sampling | 35Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL107 |
| Cell line databases/resources | cancercelllines; CVCL_RA15 |
| Encyclopedic resources | Wikidata; Q54896563 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 15 |