Cellosaurus cell line HT1080-Fluc-Puro (CVCL

Cross-references
Cell line name	HT1080-Fluc-Puro
Accession	CVCL_RA12
Resource Identification Initiative	To cite this cell line use: HT1080-Fluc-Puro (RRID:CVCL_RA12)
Comments	Population: Caucasian. Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-Puro vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL076). Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations	Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease	Fibrosarcoma (NCIt: C3043) Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0317 (HT-1080)
Sex of cell	Male
Age at sampling	35Y
Category	Cancer cell line
Cell line collections (Providers)	Imanis; CL076
Cell line databases/resources	cancercelllines; CVCL_RA12
Encyclopedic resources	Wikidata; Q54896560
Entry history
Entry creation	05-Mar-2018
Last entry update	19-Dec-2024
Version number	15