Cellosaurus HT1080-Fluc-Neo/iRFP-Puro (CVCL_RA11)
Cell line name | HT1080-Fluc-Neo/iRFP-Puro |
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Accession | CVCL_RA11 |
Resource Identification Initiative | To cite this cell line use: HT1080-Fluc-Neo/iRFP-Puro (RRID:CVCL_RA11) |
Comments | Population: Caucasian. Characteristics: Reporter cell line, transduced with both the LV-Fluc-P2A-Neo and LV-iRFP-P2A-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-iRFP-P2A-Puro encodes for iRFP linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL106). Genetic integration: Method=Transduction; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris). Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269. |
Sequence variations |
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Disease | Fibrosarcoma (NCIt: C3043) Fibrosarcoma (ORDO: Orphanet_2030) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0317 (HT-1080) |
Sex of cell | Male |
Age at sampling | 35Y |
Category | Cancer cell line |
Cross-references | |
Cell line collections (Providers) | Imanis; CL106 |
Cell line databases/resources | cancercelllines; CVCL_RA11 |
Encyclopedic resources | Wikidata; Q54896559 |
Entry history | |
Entry creation | 05-Mar-2018 |
Last entry update | 19-Dec-2024 |
Version number | 16 |