Cellosaurus WI-38/hTERT/GFP-RAF-ER (CVCL_N806)
| Cell line name | WI-38/hTERT/GFP-RAF-ER |
|---|---|
| Accession | CVCL_N806 |
| Resource Identification Initiative | To cite this cell line use: WI-38/hTERT/GFP-RAF-ER (RRID:CVCL_N806) |
| Comments | Part of: ENCODE project common cell types; tier 3. Population: Caucasian; Swedish. Characteristics: Transduced with a construct that is composed of EGFP at the N-terminus, the catalytic domain of RAF1 (with p.Tyr340Asp and p.Tyr341Asp) in the middle and the hormone-binding domain of ESR1 at the C-terminus. Protein kinase activity is activated by estrogen or analogs such as 4-hydroxy-tamoxifen. Senescence: Cells can be senesced after exposure for 72 hours with 4-hydroxy-tamoxifen. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:9829; RAF1 (Note=Catalytic domain with p.Tyr340Asp and p.Tyr341Asp). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3467; ESR1 (Note=Hormone-binding domain). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Omics: H3K4me3 ChIP-seq epigenome analysis. Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597. Cell type: Fibroblast of lung; CL=CL_0002553. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0579 (WI-38) |
| Sex of cell | Female |
| Age at sampling | 16FW |
| Category | Telomerase immortalized cell line |
| Web pages | http://genome.ucsc.edu/ENCODE/protocols/cell/human/WI38_Stam_protocol.pdf |
| Cross-references | |
| Biological sample resources | ENCODE; ENCBS070MZP
ENCODE; ENCBS157XHV ENCODE; ENCBS601ENJ ENCODE; ENCBS606ENC ENCODE; ENCBS607ENC ENCODE; ENCBS608ENC ENCODE; ENCBS715PYA ENCODE; ENCBS787LHJ |
| Encyclopedic resources | Wikidata; Q54994036 |
| Gene expression databases | GEO; GSM651580
GEO; GSM651581 GEO; GSM651582 GEO; GSM651583 GEO; GSM945215 GEO; GSM945223 GEO; GSM945245 GEO; GSM945265 |
| Entry history | |
| Entry creation | 05-Nov-2013 |
| Last entry update | 19-Dec-2024 |
| Version number | 17 |