Cellosaurus PathHunter U2OS CCKAR Activated GPCR Internalization (CVCL_KZ88)
| Cell line name | PathHunter U2OS CCKAR Activated GPCR Internalization |
|---|---|
| Synonyms | PathHunter CCKAR Activated GPCR Internalization U2OS |
| Accession | CVCL_KZ88 |
| Resource Identification Initiative | To cite this cell line use: PathHunter U2OS CCKAR Activated GPCR Internalization (RRID:CVCL_KZ88) |
| Comments | Population: Caucasian. Characteristics: PathHunter activated GPCR internalization cell lines co-express an untagged GPCR, a beta-arrestin tagged with a beta-galactosidase fragment (Enzyme Acceptor=EA) and a second beta-galactosidase fragment (ProLink=PK) localized to the endosomes. Beta-arrestin recruitment is followed by internalization of the GPCR/arrestin-EA complex in PK-tagged endosomes and allowing complementation of the PK and EA enzyme fragments thus resulting into a catalytically active enzyme that generate a chemiluminescent signal. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1570; CCKAR. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:712; ARRB2. Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
| Sequence variations |
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| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0042 (U2OS) |
| Sex of cell | Female |
| Age at sampling | 15Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | DiscoverX; 93-0650C3 |
| Encyclopedic resources | Wikidata; Q54938086 |
| Entry history | |
| Entry creation | 22-Aug-2017 |
| Last entry update | 19-Dec-2024 |
| Version number | 10 |