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Cellosaurus MEF/3T3 Tet-Off (CVCL_KS91)

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Cell line name MEF/3T3 Tet-Off
Synonyms MEF 3T3 tet-off; MEF3T3 tet-off
Accession CVCL_KS91
Resource Identification Initiative To cite this cell line use: MEF/3T3 Tet-Off (RRID:CVCL_KS91)
Comments Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Discontinued: Takara/Clontech; Catalog number 630914.
Discontinued: Takara/Clontech; Catalog number 631139.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Children:
CVCL_KU46 (MEF-T7-SRp20)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Cross-references
Encyclopedic resources Wikidata; Q54905031
Entry history
Entry creation22-Aug-2017
Last entry update19-Dec-2024
Version number11