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Cellosaurus POLK CD MSH- (CVCL_JH02)

[Text version]
Cell line name POLK CD MSH-
Synonyms Nalm-6-MSH-/TK+/-/POLK D198A,D199A
Accession CVCL_JH02
Resource Identification Initiative To cite this cell line use: POLK CD MSH- (RRID:CVCL_JH02)
Comments Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; HGNC:11830; TK1 (Note=1 of 2 alleles).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; HGNC:50800; DUX4 + HGNC; HGNC:5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).
  • Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:9183; POLK; Simple_edited; p.Asp198Ala; Zygosity=Unspecified; Note=By homologous recombination. Loss of activity (PubMed=24461735).
  • Mutation; HGNC; HGNC:9183; POLK; Simple_edited; p.Asp199Ala; Zygosity=Unspecified; Note=By homologous recombination. Loss of activity (PubMed=24461735).
  • Mutation; HGNC; HGNC:9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).
Disease Adult B acute lymphoblastic leukemia (NCIt: C9143)
Precursor B-cell acute lymphoblastic leukemia (ORDO: Orphanet_99860)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UD96 (Nalm-6-TK+/-)
Sex of cell Male
Age at sampling 19Y
Category Cancer cell line
STR profile Source(s): JCRB=JCRB1523

Markers:
AmelogeninX,Y
CSF1PO14
D5S81811,12
D7S8208,12
D13S3179,14
D16S53910,11
TH018,9
TPOX8,10
vWA15,16

Run an STR similarity search on this cell line
Publications

PubMed=24461735; DOI=10.1016/j.dnarep.2013.12.008
Sassa A., Suzuki T., Kanemaru Y., Niimi N., Fujimoto H., Katafuchi A., Gruz P., Yasui M., Gupta R.C., Johnson F., Ohta T., Honma M., Adachi N., Nohmi T.
In vivo evidence that phenylalanine 171 acts as a molecular brake for translesion DNA synthesis across benzo[a]pyrene DNA adducts by human DNA polymerase kappa.
DNA Repair 15:21-28(2014)

Cross-references
Cell line collections (Providers) JCRB; JCRB1523
Cell line databases/resources cancercelllines; CVCL_JH02
Encyclopedic resources Wikidata; Q54947711
Entry history
Entry creation15-May-2017
Last entry update19-Dec-2024
Version number16