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Cellosaurus HCT 116 KRAS(+/-) (CVCL_HD81)

[Text version]
Cell line name HCT 116 KRAS(+/-)
Synonyms KRAS (+/-) HCT116
Accession CVCL_HD81
Resource Identification Initiative To cite this cell line use: HCT 116 KRAS(+/-) (RRID:CVCL_HD81)
Comments Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; 6407; KRAS (Note=1 of 2 alleles).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
Publications

PubMed=19661383; DOI=10.1126/science.1174229
Yun J., Rago C., Cheong I., Pagliarini R., Angenendt P., Rajagopalan H., Schmidt K., Willson J.K.V., Markowitz S.D., Zhou S.-B., Diaz L.A. Jr., Velculescu V.E., Lengauer C., Kinzler K.W., Vogelstein B., Papadopoulos N.
Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells.
Science 325:1555-1559(2009)

Cross-references
Cell line collections (Providers) Horizon_Discovery; HD+104-008
Cell line databases/resources cancercelllines; CVCL_HD81
Encyclopedic resources Wikidata; Q54881959
Entry history
Entry creation01-Dec-2016
Last entry update30-Jan-2024
Version number13