Cellosaurus NIH 3T3 Duo Parental (CVCL_HC50)
| Cell line name | NIH 3T3 Duo Parental | |
|---|---|---|
| Accession | CVCL_HC50 | |
| Resource Identification Initiative | To cite this cell line use: NIH 3T3 Duo Parental (RRID:CVCL_HC50) | |
| Comments | Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites. Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO) and two copies of a TetO-ISceI-TetO construct (96 copies of TetO). Transfected with: UniProtKB; P03023; Escherichia coli lacI. Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. | |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss. | |
| Hierarchy | Parent: CVCL_0594 (NIH 3T3) Children:
| |
| Sex of cell | Male | |
| Age at sampling | Embryo | |
| Category | Spontaneously immortalized cell line | |
| Publications | PubMed=23929981; DOI=10.1126/science.1237150 PubMed=25255091; DOI=10.1038/nprot.2014.167 | |
| Cross-references | ||
| Cell line collections (Providers) | Kerafast; ENH103-FP | |
| Encyclopedic resources | Wikidata; Q54930576 | |
| Entry history | ||
| Entry creation | 01-Dec-2016 | |
| Last entry update | 29-Jun-2023 | |
| Version number | 10 | |