ID   NIH 3T3 Duo Parental
AC   CVCL_HC50
DR   Kerafast; ENH103-FP
DR   Wikidata; Q54930576
RX   PubMed=23929981;
RX   PubMed=25255091;
CC   Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites.
CC   Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO) and two copies of a TetO-ISceI-TetO construct (96 copies of TetO).
CC   Transfected with: UniProtKB; P03023; Escherichia coli lacI.
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0594 ! NIH 3T3
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 01-12-16; Last updated: 29-06-23; Version: 10
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RX   PubMed=23929981; DOI=10.1126/science.1237150;
RA   Roukos V., Voss T.C., Schmidt C.K., Lee S., Wangsa D., Misteli T.;
RT   "Spatial dynamics of chromosome translocations in living cells.";
RL   Science 341:660-664(2013).
//
RX   PubMed=25255091; DOI=10.1038/nprot.2014.167;
RA   Roukos V., Burgess R.C., Misteli T.;
RT   "Generation of cell-based systems to visualize chromosome damage and
RT   translocations in living cells.";
RL   Nat. Protoc. 9:2476-2492(2014).
//