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Cellosaurus Shh Light (CVCL_E8AK)

[Text version]
Cell line name Shh Light
Synonyms Shh-LIGHT
Accession CVCL_E8AK
Resource Identification Initiative To cite this cell line use: Shh Light (RRID:CVCL_E8AK)
Comments Characteristics: Useful for the quantitative assay of biologically active hedgehog proteins (PubMed=10984056).
Characteristics: Transfected with a GLI-responsive firefly luciferase reporter as well as a constitutive (TK promoter driven) Renilla-luciferase reporter (PubMed=10984056).
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Children:
CVCL_E8AL (SmoA1 Light)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Publications

PubMed=10984056; DOI=10.1038/35023008
Taipale J., Chen J.K., Cooper M.K., Wang B.-L., Mann R.K., Milenkovic L., Scott M.P., Beachy P.A.
Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.
Nature 406:1005-1009(2000)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1