ID   Shh Light
AC   CVCL_E8AK
SY   Shh-LIGHT
RX   PubMed=10984056;
CC   Characteristics: Useful for the quantitative assay of biologically active hedgehog proteins (PubMed=10984056).
CC   Characteristics: Transfected with a GLI-responsive firefly luciferase reporter as well as a constitutive (TK promoter driven) Renilla-luciferase reporter (PubMed=10984056).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0594 ! NIH 3T3
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 10-04-25; Last updated: 14-08-25; Version: 2
CH   CVCL_E8AL ! SmoA1 Light
//
RX   PubMed=10984056; DOI=10.1038/35023008;
RA   Taipale, Jussi
RA   Chen, James Kenneth
RA   Cooper, Michael K.
RA   Wang, Bao-Lin
RA   Mann, Randall Krage
RA   Milenkovic, Ljiljana
RA   Scott, Matthew P.
RA   Beachy, Philip Arden
RT   "Effects of oncogenic mutations in Smoothened and Patched can be
RT   reversed by cyclopamine.";
RL   Nature 406:1005-1009(2000).
//