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Cellosaurus iLite TNF-alpha Assay Ready Cells (CVCL_E7RU)

[Text version]
Cell line name iLite TNF-alpha Assay Ready Cells
Synonyms KJL-Luc
Accession CVCL_E7RU
Resource Identification Initiative To cite this cell line use: iLite TNF-alpha Assay Ready Cells (RRID:CVCL_E7RU)
Comments Population: Caucasian.
Characteristics: Genetically engineered and optimized to respond to TNF-alpha resulting in the specific expression of firefly luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla luciferase reporter gene construct, under control of a constitutive promoter (Svar Life Science=BM3044).
Characteristics: The luciferase reporter gene is under the control of 5 tandem copies of the NF-kappaB response element and the Renilla luciferase is under the control of a constitutive minimal TK promoter (PubMed=21910993).
Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110)
Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E7RV (KK-Luc)
Sex of cell Female
Age at sampling 53Y
Category Cancer cell line
Web pages Provider; Svar Life Science; BM3044; https://www.svarlifescience.com/products/bm3044
Publications

PubMed=21910993; DOI=10.1016/j.jim.2011.08.022
Lallemand C., Kavrochorianou N., Steenholdt C., Bendtzen K., Ainsworth M.A., Meritet J.-F., Blanchard B., Lebon P., Taylor P., Charles P., Alzabin S., Tovey M.G.
Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFalpha antagonists.
J. Immunol. Methods 373:229-239(2011)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1