ID   iLite TNF-alpha Assay Ready Cells
AC   CVCL_E7RU
SY   KJL-Luc
RX   PubMed=21910993;
WW   Provider; Svar Life Science; BM3044; https://www.svarlifescience.com/products/bm3044
CC   Population: Caucasian.
CC   Characteristics: Genetically engineered and optimized to respond to TNF-alpha resulting in the specific expression of firefly luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla luciferase reporter gene construct, under control of a constitutive promoter (Svar Life Science=BM3044).
CC   Characteristics: The luciferase reporter gene is under the control of 5 tandem copies of the NF-kappaB response element and the Renilla luciferase is under the control of a constitutive minimal TK promoter (PubMed=21910993).
CC   Sequence variation: Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
DI   NCIt; C9110; Blast phase chronic myelogenous leukemia, BCR-ABL1 positive
DI   ORDO; Orphanet_521; Chronic myeloid leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E7RV ! KK-Luc
SX   Female
AG   53Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=21910993; DOI=10.1016/j.jim.2011.08.022;
RA   Lallemand C., Kavrochorianou N., Steenholdt C., Bendtzen K.,
RA   Ainsworth M.A., Meritet J.-F., Blanchard B., Lebon P., Taylor P.,
RA   Charles P., Alzabin S., Tovey M.G.;
RT   "Reporter gene assay for the quantification of the activity and
RT   neutralizing antibody response to TNFalpha antagonists.";
RL   J. Immunol. Methods 373:229-239(2011).
//