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Cellosaurus MCF-7/B2 (CVCL_E7HX)

[Text version]
Cell line name MCF-7/B2
Accession CVCL_E7HX
Resource Identification Initiative To cite this cell line use: MCF-7/B2 (RRID:CVCL_E7HX)
Comments Population: Caucasian.
Characteristics: Upon phosphatidyl inositol-3 phosphate (PIP3) production, the recruitment of Luc-Akt-PH to the plasma membrane results in a bioluminescence resonance energy transfer (BRET) between the luciferase and the YFP (PubMed=24647478).
Characteristics: Transfected with a Luc-Akt-PH construct where the PH domain of mouse Akt1 is fused to Renilla luciferase as well as a construct containing YFP fused with a membrane targeting sequence (EYFP-Mem) (PubMed=24647478).
Genetic integration: Method=Transfection; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transfection; Gene=MGI; MGI:87986; Akt1 (Note=With p.Lys111_Ala480del = PH domain).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E7HW (MCF-7/Luc-Akt-PH)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
Web pages Provider; Inserm Transfert; RT00733; https://technology-offers.inserm-transfert.com/offer/a-breast-cancer-cell-line-expressing-a-bret-biosensor-to-monitor-pi-3-kinase-activity-in-real-time/
Publications

PubMed=24647478; DOI=10.1371/journal.pone.0092737; PMCID=PMC3960261
Kuo M.-S., Auriau J., Pierre-Eugene C., Issad T.
Development of a human breast-cancer derived cell line stably expressing a bioluminescence resonance energy transfer (BRET)-based phosphatidyl inositol-3 phosphate (PIP3) biosensor.
PLoS ONE 9:e92737.1-e92737.12(2014)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1