ID   MCF-7/B2
AC   CVCL_E7HX
RX   PubMed=24647478;
WW   Provider; Inserm Transfert; RT00733; https://technology-offers.inserm-transfert.com/offer/a-breast-cancer-cell-line-expressing-a-bret-biosensor-to-monitor-pi-3-kinase-activity-in-real-time/
CC   Population: Caucasian.
CC   Characteristics: Upon phosphatidyl inositol-3 phosphate (PIP3) production, the recruitment of Luc-Akt-PH to the plasma membrane results in a bioluminescence resonance energy transfer (BRET) between the luciferase and the YFP (PubMed=24647478).
CC   Characteristics: Transfected with a Luc-Akt-PH construct where the PH domain of mouse Akt1 is fused to Renilla luciferase as well as a construct containing YFP fused with a membrane targeting sequence (EYFP-Mem) (PubMed=24647478).
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:87986; Akt1 (Note=With p.Lys111_Ala480del = PH domain).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
DI   NCIt; C4194; Invasive breast carcinoma of no special type
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E7HW ! MCF-7/Luc-Akt-PH
SX   Female
AG   69Y
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=24647478; DOI=10.1371/journal.pone.0092737; PMCID=PMC3960261;
RA   Kuo M.-S., Auriau J., Pierre-Eugene C., Issad T.;
RT   "Development of a human breast-cancer derived cell line stably
RT   expressing a bioluminescence resonance energy transfer (BRET)-based
RT   phosphatidyl inositol-3 phosphate (PIP3) biosensor.";
RL   PLoS ONE 9:e92737.1-e92737.12(2014).
//