Cellosaurus MEF OGT(F/Y) mER-Cre-2A-GFP (CVCL_E6HN)
| Cell line name | MEF OGT(F/Y) mER-Cre-2A-GFP |
|---|---|
| Accession | CVCL_E6HN |
| Resource Identification Initiative | To cite this cell line use: MEF OGT(F/Y) mER-Cre-2A-GFP (RRID:CVCL_E6HN) |
| Comments | Characteristics: Transduced with a CreERT2-2A-GFP construct. In the absence of 4-hydroxytamoxifen (4-OHT), the CreERT2 chimera is retained in the cytoplasm. In the presence of 4-OHT the chimera dimerizes and translocates to the nucleus recombining the loxP sites, resulting in an Ogt null cell (PubMed=20926391). Knockout cell: Method=KO mouse; MGI; MGI:1339639; Ogt (Note=Flanked with two loXP sites). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2). Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Mus musculus (Mouse) (NCBI Taxonomy: 10090) |
| Sex of cell | Sex unspecified |
| Age at sampling | Embryo |
| Category | Finite cell line |
| Web pages | Provider; JHTV; C17974; https://jhu.technologypublisher.com/technology/52634 |
| Publications | PubMed=20926391; DOI=10.1074/jbc.M110.131102; PMCID=PMC2998145 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |