ID   MEF OGT(F/Y) mER-Cre-2A-GFP
AC   CVCL_E6HN
RX   PubMed=20926391;
WW   Provider; JHTV; C17974; https://jhu.technologypublisher.com/technology/52634
CC   Characteristics: Transduced with a CreERT2-2A-GFP construct. In the absence of 4-hydroxytamoxifen (4-OHT), the CreERT2 chimera is retained in the cytoplasm. In the presence of 4-OHT the chimera dimerizes and translocates to the nucleus recombining the loxP sites, resulting in an Ogt null cell (PubMed=20926391).
CC   Knockout cell: Method=KO mouse; MGI; MGI:1339639; Ogt (Note=Flanked with two loXP sites).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Sex unspecified
AG   Embryo
CA   Finite cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=20926391; DOI=10.1074/jbc.M110.131102; PMCID=PMC2998145;
RA   Kazemi Z., Chang H., Haserodt S., McKen C., Zachara N.E.;
RT   "O-linked beta-N-acetylglucosamine (O-GlcNAc) regulates stress-induced
RT   heat shock protein expression in a GSK-3beta-dependent manner.";
RL   J. Biol. Chem. 285:39096-39107(2010).
//