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Cellosaurus 1D3 [Mouse hybridoma against bovine CYCS] (CVCL_E5SJ)

[Text version]
Cell line name 1D3 [Mouse hybridoma against bovine CYCS]
Accession CVCL_E5SJ
Resource Identification Initiative To cite this cell line use: 1D3 [Mouse hybridoma against bovine CYCS] (RRID:CVCL_E5SJ)
Comments Monoclonal antibody isotype: IgG1, kappa.
Monoclonal antibody target: UniProtKB; P62894; Bovine CYCS (Note=Recognizes a conformational change that happens early in apoptosis and in necrosis. Also reacts with mouse and rat).
Anecdotal: Because of the loss of this hybridoma, a new recombinant mAB (R1D3) was created de novo using the sequence of remaining stocks of the mAB 1D3 (PubMed=36378644).
Miscellaneous: This hybridoma was lost in a defective liquid nitrogen tank (PubMed=36378644).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_4032 (P3X63Ag8.653)
Category Hybridoma
Web pages Info; -; -; https://phys.org/news/2023-03-scientists-resurrect-dead-antibody-protein.html
Publications

PubMed=10090746; DOI=10.1021/bi9809268
Jemmerson R., Liu J., Hausauer D.L., Lam K.-P., Mondino A., Nelson R.D.
A conformational change in cytochrome c of apoptotic and necrotic cells is detected by monoclonal antibody binding and mimicked by association of the native antigen with synthetic phospholipid vesicles.
Biochemistry 38:3599-3609(1999)

PubMed=36378644; DOI=10.1073/pnas.2213432119; PMCID=PMC9704708
Tomasina F., Martinez J., Zeida A., Chiribao M.L., Demicheli V., Correa A., Quijano C., Castro L., Carnahan R.H., Vinson P.N., Goff M., Cooper T., McDonald W.H., Castellana N., Hannibal L., Morse P.T., Wan J.-M., Huttemann M., Jemmerson R., Piacenza L., Radi R.
De novo sequencing and construction of a unique antibody for the recognition of alternative conformations of cytochrome c in cells.
Proc. Natl. Acad. Sci. U.S.A. 119:e2213432119.1-e2213432119.12(2022)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1