ID   1D3 [Mouse hybridoma against bovine CYCS]
AC   CVCL_E5SJ
RX   PubMed=10090746;
RX   PubMed=36378644;
WW   Info; -; -; https://phys.org/news/2023-03-scientists-resurrect-dead-antibody-protein.html
CC   Monoclonal antibody isotype: IgG1, kappa.
CC   Monoclonal antibody target: UniProtKB; P62894; Bovine CYCS (Note=Recognizes a conformational change that happens early in apoptosis and in necrosis. Also reacts with mouse and rat).
CC   Anecdotal: Because of the loss of this hybridoma, a new recombinant mAB (R1D3) was created de novo using the sequence of remaining stocks of the mAB 1D3 (PubMed=36378644).
CC   Miscellaneous: This hybridoma was lost in a defective liquid nitrogen tank (PubMed=36378644).
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_4032 ! P3X63Ag8.653
CA   Hybridoma
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=10090746; DOI=10.1021/bi9809268;
RA   Jemmerson R., Liu J., Hausauer D.L., Lam K.-P., Mondino A., Nelson R.D.;
RT   "A conformational change in cytochrome c of apoptotic and necrotic
RT   cells is detected by monoclonal antibody binding and mimicked by
RT   association of the native antigen with synthetic phospholipid
RT   vesicles.";
RL   Biochemistry 38:3599-3609(1999).
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RX   PubMed=36378644; DOI=10.1073/pnas.2213432119; PMCID=PMC9704708;
RA   Tomasina F., Martinez J., Zeida A., Chiribao M.L., Demicheli V.,
RA   Correa A., Quijano C., Castro L., Carnahan R.H., Vinson P.N., Goff M.,
RA   Cooper T., McDonald W.H., Castellana N., Hannibal L., Morse P.T.,
RA   Wan J.-M., Huttemann M., Jemmerson R., Piacenza L., Radi R.;
RT   "De novo sequencing and construction of a unique antibody for the
RT   recognition of alternative conformations of cytochrome c in cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 119:e2213432119.1-e2213432119.12(2022).
//