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Cellosaurus EMC-G5 (CVCL_E4IF)

[Text version]
Cell line name EMC-G5
Synonyms G5
Accession CVCL_E4IF
Resource Identification Initiative To cite this cell line use: EMC-G5 (RRID:CVCL_E4IF)
Comments Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1069; BMP2.
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Omics: Proteome analysis by 2D-DE/MS.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_1977 (CHO-DXB11)
Children:
CVCL_E4IG (EMC-G5 G5P.7A 3C9)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=18240028; DOI=10.1007/s12033-008-9039-6
Doolan P., Melville M.W., Gammell P., Sinacore M.S., Meleady P., McCarthy K., Francullo L., Leonard M.W., Charlebois T.S., Clynes M.
Transcriptional profiling of gene expression changes in a PACE-transfected CHO DUKX cell line secreting high levels of rhBMP-2.
Mol. Biotechnol. 39:187-199(2008)

PubMed=18546152; DOI=10.1002/pmic.200700854
Meleady P., Henry M., Gammell P., Doolan P., Sinacore M.S., Melville M.W., Francullo L., Leonard M.W., Charlebois T.S., Clynes M.
Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol.
Proteomics 8:2611-2624(2008)

Patent=US8039231
Luan Y.-T., Wang W.-G., Nyberg G.B., Gomes J.M., Drapeau D., Cardoza Stanek T.
Methods for enhanced production of bone morphogenetic proteins.
Patent number US8039231, 18-Oct-2011

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1