ID   EMC-G5
AC   CVCL_E4IF
SY   G5
RX   Patent=US8039231;
RX   PubMed=18240028;
RX   PubMed=18546152;
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1069; BMP2.
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
CC   Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Transformant: NCIt; C44386; Gamma radiation.
CC   Omics: Proteome analysis by 2D-DE/MS.
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_1977 ! CHO-DXB11
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 19-12-24; Last updated: 19-12-24; Version: 1
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RX   Patent=US8039231;
RA   Luan Y.-T., Wang W.-G., Nyberg G.B., Gomes J.M., Drapeau D.,
RA   Cardoza Stanek T.;
RT   "Methods for enhanced production of bone morphogenetic proteins.";
RL   Patent number US8039231, 18-Oct-2011.
//
RX   PubMed=18240028; DOI=10.1007/s12033-008-9039-6;
RA   Doolan P., Melville M.W., Gammell P., Sinacore M.S., Meleady P.,
RA   McCarthy K., Francullo L., Leonard M.W., Charlebois T.S., Clynes M.;
RT   "Transcriptional profiling of gene expression changes in a
RT   PACE-transfected CHO DUKX cell line secreting high levels of rhBMP-2.";
RL   Mol. Biotechnol. 39:187-199(2008).
//
RX   PubMed=18546152; DOI=10.1002/pmic.200700854;
RA   Meleady P., Henry M., Gammell P., Doolan P., Sinacore M.S., Melville M.W.,
RA   Francullo L., Leonard M.W., Charlebois T.S., Clynes M.;
RT   "Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity
RT   following co-expression of PACEsol.";
RL   Proteomics 8:2611-2624(2008).
//