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Cellosaurus BJ SiPS-D TH-TdTomato ATP10B KO clone #4 (CVCL_E3SY)

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Cell line name BJ SiPS-D TH-TdTomato ATP10B KO clone #4
Synonyms TH-TdTomato BJ SiPS ATP10B KO clone #4
Accession CVCL_E3SY
Resource Identification Initiative To cite this cell line use: BJ SiPS-D TH-TdTomato ATP10B KO clone #4 (RRID:CVCL_E3SY)
Comments Population: Caucasian.
Characteristics: Fluorescent (tdTomato) reporter cell line for TH expression. Using CRISPR/Cas9 a P2A-tdTomato construct was introduced in the C-terminal end of TH (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:13543; ATP10B.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C8FN (BJ SiPS-D TH-TdTomato)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Publications

DOI=10.1101/2024.07.09.602700
Sanchiz-Calvo M., Coccia E., Cawthorne C., Parfitt G.M., Van Laere K., Torre-Muruzabal T., Cabezudo D., Tsafaras G., Cascalho A.C.C., Van den Haute C., Vangheluwe P., Blanchard J., Bentea E., Baekelandt V.
Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits.
bioRxiv 2024:07.09.602700-07.09.602700(2024)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1