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Cellosaurus BJ SiPS-D TH-TdTomato (CVCL_C8FN)

[Text version]
Cell line name BJ SiPS-D TH-TdTomato
Synonyms TH-TdTomato BJ SiPS Reporter line; BJ-SIPS WT TH
Accession CVCL_C8FN
Resource Identification Initiative To cite this cell line use: BJ SiPS-D TH-TdTomato (RRID:CVCL_C8FN)
Comments Population: Caucasian.
Characteristics: Fluorescent (tdTomato) reporter cell line for TH expression. Using CRISPR/Cas9 a P2A-tdTomato construct was introduced in the C-terminal end of TH (PubMed=31902706).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_X741 (BJ SiPS-D)
Children:
CVCL_D5YD (BJ SiPS-D TH-TdTomato DJ1 HET KO)CVCL_D5YE (BJ SiPS-D TH-TdTomato DJ1 HOM KO)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Publications

PubMed=31902706; DOI=10.1016/j.stemcr.2019.12.005; PMCID=PMC6962705
Ahfeldt T., Ordureau A., Bell C., Sarrafha L., Sun C.-C., Piccinotti S., Grass T., Parfitt G.M., Paulo J.A., Yanagawa F., Uozumi T., Kiyota Y., Harper J.W., Rubin L.L.
Pathogenic pathways in early-onset autosomal recessive Parkinson's disease discovered using isogenic human dopaminergic neurons.
Stem Cell Reports 14:75-90(2020)

Cross-references
Encyclopedic resources Wikidata; Q123030815
Entry history
Entry creation29-Jun-2023
Last entry update10-Sep-2024
Version number3