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Cellosaurus CHO-K1 MUC1-IgG (CVCL_E3IX)

[Text version]
Cell line name CHO-K1 MUC1-IgG
Synonyms CHO MUC1
Accession CVCL_E3IX
Resource Identification Initiative To cite this cell line use: CHO-K1 MUC1-IgG (RRID:CVCL_E3IX)
Comments Characteristics: The transfected MUC1 construct contains the first 16 tandem repeats of the extracellular domain of MUC1 followed by an enterokinase cleavage site (DDDDK) fusef to exon 1-3 of mouse IgG2a Fc (PubMed=16319059).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7508; MUC1 (Note=With p.Ala381_Leu1255del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Children:
CVCL_E3IY (CHO-K1 MUC1-IgG ST6GALNAC1)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=12950230; DOI=10.1042/bj20031130; PMCID=PMC1223800
Backstrom M., Link T., Olson F.J., Karlsson H., Graham R.A., Picco G., Burchell J.M., Taylor-Papadimitriou J., Noll T., Hansson G.C.
Recombinant MUC1 mucin with a breast cancer-like O-glycosylation produced in large amounts in Chinese-hamster ovary cells.
Biochem. J. 376:677-686(2003)

PubMed=16319059; DOI=10.1074/jbc.M511826200
Sewell R., Backstrom M., Dalziel M., Gschmeissner S., Karlsson H., Noll T., Gatgens J., Clausen H., Hansson G.C., Burchell J.M., Taylor-Papadimitriou J.
The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
J. Biol. Chem. 281:3586-3594(2006)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1