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Cellosaurus HeLa-dCas9 agDD-GFP (CVCL_E3BI)

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Cell line name HeLa-dCas9 agDD-GFP
Accession CVCL_E3BI
Resource Identification Initiative To cite this cell line use: HeLa-dCas9 agDD-GFP (RRID:CVCL_E3BI)
Comments Population: African American.
Characteristics: Stably expresses agDD-GFP which consists of an aggregating destabilizing domain (AgDD) fused to the N-terminal of Superfolder GFP.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=FPbase; B4SOW; Superfolder GFP (Note=sfGFP, superfolding variant of GFP).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E3BH (HeLa-dCas9)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Entry history
Entry creation10-Sep-2024
Last entry update19-Dec-2024
Version number2