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Cellosaurus HeLa-dCas9 (CVCL_E3BH)

[Text version]
Cell line name HeLa-dCas9
Accession CVCL_E3BH
Resource Identification Initiative To cite this cell line use: HeLa-dCas9 (RRID:CVCL_E3BH)
Comments Population: African American.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_E3BI (HeLa-dCas9 agDD-GFP)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=23849981; DOI=10.1016/j.cell.2013.06.044; PMCID=PMC3770145
Gilbert L.A., Larson M.H., Morsut L., Liu Z., Brar G.A., Torres S.E., Stern-Ginossar N., Brandman O., Whitehead E.H., Doudna J.A., Lim W.A., Weissman J.S., Qi L.S.
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes.
Cell 154:442-451(2013)

Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1