Cellosaurus EPC-EC-Vip-C7 (CVCL_E3AJ)
| Cell line name | EPC-EC-Vip-C7 |
|---|---|
| Accession | CVCL_E3AJ |
| Resource Identification Initiative | To cite this cell line use: EPC-EC-Vip-C7 (RRID:CVCL_E3AJ) |
| Comments | Group: Fish cell line. Problematic cell line: Misidentified. Grand-parent cell line (EPC) was originally deposited as a carp (Cyprinus carpio) cell line, is in fact a fathead minnow (Pimephales promelas) cell line. Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against stat2 and against mEGFP. mEGFP-deficient cells were isolated (PubMed=38951796). Knockout cell: Method=CRISPR/Cas9; UniProtKB; A0A096ZN79; Viperin (Vig1). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Cell type: Epithelial cell; CL=CL_0000066. |
| Species of origin | Pimephales promelas (Fathead minnow) (NCBI Taxonomy: 90988) |
| Hierarchy | Parent: CVCL_E3AG (EPC-EC) |
| Sex of cell | Sex unspecified |
| Age at sampling | Age unspecified |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=38951796; DOI=10.1186/s12864-024-10566-x; PMCID=PMC11218377 |
| Entry history | |
| Entry creation | 10-Sep-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 1 |