ID   EPC-EC-Vip-C7
AC   CVCL_E3AJ
RX   PubMed=38951796;
CC   Group: Fish cell line.
CC   Problematic cell line: Misidentified. Grand-parent cell line (EPC) was originally deposited as a carp (Cyprinus carpio) cell line, is in fact a fathead minnow (Pimephales promelas) cell line.
CC   Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against stat2 and against mEGFP. mEGFP-deficient cells were isolated (PubMed=38951796).
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; A0A096ZN79; Viperin (Vig1).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Cell type: Epithelial cell; CL=CL_0000066.
OX   NCBI_TaxID=90988; ! Pimephales promelas (Fathead minnow)
HI   CVCL_E3AG ! EPC-EC
SX   Sex unspecified
AG   Age unspecified
CA   Spontaneously immortalized cell line
DT   Created: 10-09-24; Last updated: 10-09-24; Version: 1
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RX   PubMed=38951796; DOI=10.1186/s12864-024-10566-x; PMCID=PMC11218377;
RA   Chaumont L., Jouneau L., Huetz F., van Muilekom D.R., Peruzzi M.,
RA   Raffy C., Le Hir J., Minke J., Boudinot P., Collet B.;
RT   "Unexpected regulatory functions of cyprinid viperin on inflammation
RT   and metabolism.";
RL   BMC Genomics 25:650.1-650.28(2024).
//