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Cellosaurus HEK293T AGA KO (CVCL_E2QF)

[Text version]
Cell line name HEK293T AGA KO
Synonyms HEK293T-AGA-knockout
Accession CVCL_E2QF
Resource Identification Initiative To cite this cell line use: HEK293T AGA KO (RRID:CVCL_E2QF)
Comments Knockout cell: Method=CRISPR/Cas9; HGNC; 318; AGA.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Children:
CVCL_E2QH (HEK293T AGA/SLC35A1 DKO)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=27983621; DOI=10.3390/cells5040045; PMCID=PMC5187529
Kapahnke M., Banning A., Tikkanen R.
Random splicing of several exons caused by a single base change in the target exon of CRISPR/Cas9 mediated gene knockout.
Cells 5:45.1-45.12(2016)

PubMed=34069698; DOI=10.3390/cells10051259; PMCID=PMC8160606
Banning A., Zakrzewicz A., Chen X., Gray S.J., Tikkanen R.
Knockout of the CMP-sialic acid transporter SLC35A1 in human cell lines increases transduction efficiency of adeno-associated virus 9: implications for gene therapy potency assays.
Cells 10:1259.1-1259.14(2021)

Entry history
Entry creation10-Sep-2024
Last entry update10-Sep-2024
Version number1