ID   HEK293T AGA KO
AC   CVCL_E2QF
SY   HEK293T-AGA-knockout
RX   PubMed=27983621;
RX   PubMed=34069698;
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 318; AGA.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 10-09-24; Last updated: 10-09-24; Version: 1
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RX   PubMed=27983621; DOI=10.3390/cells5040045; PMCID=PMC5187529;
RA   Kapahnke M., Banning A., Tikkanen R.;
RT   "Random splicing of several exons caused by a single base change in
RT   the target exon of CRISPR/Cas9 mediated gene knockout.";
RL   Cells 5:45.1-45.12(2016).
//
RX   PubMed=34069698; DOI=10.3390/cells10051259; PMCID=PMC8160606;
RA   Banning A., Zakrzewicz A., Chen X., Gray S.J., Tikkanen R.;
RT   "Knockout of the CMP-sialic acid transporter SLC35A1 in human cell
RT   lines increases transduction efficiency of adeno-associated virus 9:
RT   implications for gene therapy potency assays.";
RL   Cells 10:1259.1-1259.14(2021).
//