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Cellosaurus G16L1.1c8 (CVCL_DC17)

[Text version]
Cell line name G16L1.1c8
Accession CVCL_DC17
Resource Identification Initiative To cite this cell line use: G16L1.1c8 (RRID:CVCL_DC17)
Comments Biotechnology: Cell line developed for CALUX (Chemically Activated LUciferase eXpression) bioassay. CALUX is used for rapid screening of samples for the presence of dioxin/TCDD-like compounds and AhR agonists/antagonists.
Characteristics: Acts through the activation of a luciferase reporter gene under the control of dioxin-response elements (DREs).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Transformant: ChEBI; CHEBI_50102; N-methyl-N-nitrosourea (NMU).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Disease Guinea pig colon adenocarcinoma (NCIt: C134563)
Species of origin Cavia porcellus (Guinea pig) (NCBI Taxonomy: 10141)
Hierarchy Parent: CVCL_3463 (GPC-16)
Sex of cell Female
Age at sampling Adult
Category Cancer cell line
Publications

PubMed=8812265; DOI=10.1006/faat.1996.0056
Garrison P.M., Tullis K., Aarts J.M.M.J.G., Brouwer A., Giesy J.P., Denison M.S.
Species-specific recombinant cell lines as bioassay systems for the detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals.
Fundam. Appl. Toxicol. 30:194-203(1996)

Patent=US5854010
Denison M.S., Brouwer A., Clark G.C.
Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor.
Patent number US5854010, 29-Dec-1998

PubMed=18969542; DOI=10.1016/j.talanta.2004.05.032
Denison M.S., Zhao B., Baston D.S., Clark G.C., Murata H., Han D.
Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals.
Talanta 63:1123-1133(2004)

PubMed=21394221; DOI=10.1007/s11426-010-0142-8; PMCID=PMC3049243
Zhao B., Baston D.S., Khan E., Sorrentino C., Denison M.S.
Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds.
Sci. China Chem. 53:1010-1016(2010)

PubMed=26366531; DOI=10.1021/acs.est.5b02906; PMCID=PMC4772899
Brennan J.C., He G.-C., Tsutsumi T., Zhao J., Wirth E., Fulton M.H., Denison M.S.
Development of species-specific Ah receptor-responsive third generation CALUX cell lines with enhanced responsiveness and improved detection limits.
Environ. Sci. Technol. 49:11903-11912(2015)

Cross-references
Encyclopedic resources Wikidata; Q54835369
Entry history
Entry creation13-Jul-2016
Last entry update19-Dec-2024
Version number11