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Cellosaurus WAe009-A-W (CVCL_D6K5)

[Text version]
Cell line name WAe009-A-W
Synonyms H9 NGN2 B6; UOWe010-A-W
Accession CVCL_D6K5
Resource Identification Initiative To cite this cell line use: WAe009-A-W (RRID:CVCL_D6K5)
Comments From: University of Wollongong; Wollongong; Australia.
Characteristics: Using CRISPR/Cas9 a stably integrated doxycycline-inducible NEUROG2/NGN2 construct (LoxP-EF1A-Bsd-mApple-LoxP-CAG-rtTA3G-NEUROG2-TRe3G was introduced in the CLYBL locus (PubMed=38244534).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; 13805; NEUROG2.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; T5UGO; mApple (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=38244534; DOI=10.1016/j.scr.2024.103312
Miellet S., St Clair-Glover M., Maddock M., Dottori M.
Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus.
Stem Cell Res. 75:103312-103312(2024)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-W
Biological sample resources BioSamples; SAMEA114312888
Encyclopedic resources Wikidata; Q127384686
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2