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Cellosaurus WAe009-A-1A (CVCL_D6K3)

[Text version]
Cell line name WAe009-A-1A
Synonyms SMUDHe010-A-1A
Accession CVCL_D6K3
Resource Identification Initiative To cite this cell line use: WAe009-A-1A (RRID:CVCL_D6K3)
Comments From: Southern Medical University; Guangzhou; China.
Characteristics: Using CRISPR/Cas9 a Brainbow-2.1 R cassette that includes nuclear GFP (nGFP), EYFP, RFP (red) and membrane-bound mCerulean was introduced in the AAVS1 safe harbor locus. Stochastic recombination using incompatible lox sites can produce by inversions and excisions four different mixtures of fluorescent proteins (PubMed=38507883).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; J2JWA; mCerulean (Note=Bright cyan fluorescent protein derived from GFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=38507883; DOI=10.1016/j.scr.2024.103383
Luo Y.-Y., Zheng W., Zhong Y.-D., Liu H.-T., Yu J., Qiu B.-Y., Liu J., Yang B.
Generation of a human embryonic stem cell line (SMUDHe010-A-1A) carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated homologous recombination.
Stem Cell Res. 77:103383-103383(2024)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-1A
Biological sample resources BioSamples; SAMEA114492926
Encyclopedic resources Wikidata; Q127384681
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2