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Cellosaurus A20-eGFP-Neo/Fluc-Puro (CVCL_D5KA)

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Cell line name A20-eGFP-Neo/Fluc-Puro
Accession CVCL_D5KA
Resource Identification Initiative To cite this cell line use: A20-eGFP-Neo/Fluc-Puro (RRID:CVCL_D5KA)
Comments Characteristics: Reporter cell line, transfected with both the LV-eGFP-P2A-Neo and LV-Fluc-P2A-Puro vectors. LV-eGFP-P2A-Neo encodes for eGFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter and LV-Fluc-P2A-Puro encodes for luciferase linked via a P2A cleavage peptide to the pac gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL152).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P08659; Firefly luciferase.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Cell type: B-cell; CL=CL_0000236.
Disease Mouse reticulum cell sarcoma (NCIt: C134780)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cAnN.
Hierarchy Parent: CVCL_1940 (A20)
Sex of cell Sex unspecified
Age at sampling >15M
Category Cancer cell line
Cross-references
Cell line collections (Providers) Imanis; CL152
Entry history
Entry creation02-May-2024
Last entry update02-May-2024
Version number1