ID   A20-eGFP-Neo/Fluc-Puro
AC   CVCL_D5KA
DR   Imanis; CL152
DR   Wikidata; Q127378812
CC   Characteristics: Reporter cell line, transfected with both the LV-eGFP-P2A-Neo and LV-Fluc-P2A-PuroR vectors. LV-eGFP-P2A-Neo encodes for eGFP linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter and LV-Fluc-P2A-PuroR encodes for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL152).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Cell type: B-cell; CL=CL_0000236.
CC   Breed/subspecies: BALB/cAnN.
DI   NCIt; C134780; Mouse reticulum cell sarcoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_1940 ! A20
SX   Sex unspecified
AG   >15M
CA   Cancer cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
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